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yacF [2019-07-19 11:11:53]
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yacF [2019-07-19 11:11:53]

putative tRNA-dihydrouridine synthase B
Locus
BSU_00810
Isoelectric point
7.87
Molecular weight
36.91 kDa
Protein length
333 aa Sequence Blast
Gene length
1002 bp Sequence Blast
Function
tRNA maturation
Product
putative tRNA-dihydrouridine synthase B
Essential
no
Synonyms
dusB,dus1
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

    • Gene

    Coordinates
    87,634 → 88,635

    The protein

    Catalyzed reaction/ biological activity

  • 5,6-dihydrouridine in tRNA + NAD+ --> uridine in tRNA + H+ + NADH (according to UniProt)
  • 5,6-dihydrouridine in tRNA + NADP+ --> uridine in tRNA + H+ + NADPH

    • Protein family

  • Dus family (with YfjN, according to UniProt)

    • Paralogous protein(s)

  • YfjN

    • Cofactors

  • FMN or FAD PubMed

    • Structure

  • 1VHN (from Thermotoga maritima, 35% identity, 54% similarity) PubMed

    • Expression and Regulation

    Operons

    Genes
    pabB-pabA-pabC-sul-folB-folK-yazB-yacF-lysS
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulation

  • translation repressed by tryptophan (MtrB) PubMed
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    Additional information

  • the mRNA is very stable (> 15 min) PubMed

    • Biological materials

    Mutant

  • MGNA-B927 (yacF::erm), available at the NBRP B. subtilis, Japan
  • BKE00810 (ΔyacF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTTTCTCCTCCTTTCA, downstream forward: _UP4_TAATACTCACCTCTATTTGC
  • BKK00810 (ΔyacF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTTTCTCCTCCTTTCA, downstream forward: _UP4_TAATACTCACCTCTATTTGC

    • References

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